The Jaggar laboratory investigates the physiological regulation of arterial contractility and systemic blood pressure by ion channels expressed in smooth muscle and endothelial cells. We also examine pathological alterations in ion channels that are associated with cardiovascular diseases, including hypertension and stroke. Ion channels that we currently study include transient receptor potential (TRP), large-conductance calcium-activated potassium (BK) and transmembrane 16A (TMEM16A). We use a broad range of molecular, biochemical, cellular, and functional approaches. Methods used in the laboratory include novel inducible, cell-specific gene knockout and cardiovascular disease models, RT-PCR, Western blotting, biotinylation, FRET, RNAi, co-IP, immunofluorescence, patch-clamp electrophysiology, membrane potential recording, intracellular calcium imaging, arterial myography and blood pressure telemetry.
Jonathan H. Jaggar, Ph.D.